Genetic characteristics of 22 Y-STR loci in Koreans.

Park, Myung Jin; Lee, Hwan Young; Kim, Na Young; Sim, Jeong Eun; Yang, Woo Ick; Cho, Sang Ho; Shin, Kyoung Jin

Original article

Korean Journal of Legal Medicine 2007;31(2):162-170.
Published online November 30, 2007.

Genetic characteristics of 22 Y-STR loci in Koreans.

Myung Jin Park, Hwan Young Lee, Na Young Kim, Jeong Eun Sim, Woo Ick Yang, Sang Ho Cho, Kyoung Jin Shin

¹Department of Forensic Medicine, Yonsei University College of Medicine, Seoul, Korea. kjshin@yuhs.ac
²Human Identification Research Center, Yonsei University, Seoul, Korea.

Abstract

To construct a Korean Y-chromosomal STR database for 22 Y-STRs (DYS19, DYS385, DYS388, DYS389I/II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438, DYS439, DYS446, DYS447, DYS448, DYS449, DYS456, DYS458, DYS464, DYS635, and GATA H4.1), 708 DNA samples from unrelated Korean males were analyzed using three multiplex PCR systems. During analysis, thirty two microvariant alleles were observed at DYS449, DYS458, and DYS464, and duplicated alleles were occurred once each for DYS19, DYS390, and DYS447. In haplotype analysis for the 22 Y-STRs, a total of 693 different haplotypes were observed with overall haplotype diversity of 0.9999, and of these, 680 haplotypes were unique. By combining each marker for the extended SWGDAM haplotype, DYS458 was the most informative marker. In addition, the diversity of combined haplotypes of DYS447, DYS458, DYS635, GATA H4.1, and the SWGDAM Y-STR loci was comparable to haplotypes of 17 loci in the AmpFlSTR(R) Yfiler(TM) kit.

Key Words: Y chromosome, STR, extended SWGDAM haplotype, AmpFlSTR(R) Yfiler(TM), microvariant allele, duplicated allele, Koreans