Analysis of Polymorphism of Penta E and Penta D in Korean.

Zhang, Yong Ji; Kim, Ki Beom; Kim, Mi Jin; Lee, Jung Bin

Original article

Korean Journal of Legal Medicine 2003;27(1):45-50.
Published online May 31, 2003.

Analysis of Polymorphism of Penta E and Penta D in Korean.

Yong Ji Zhang, Ki Beom Kim, Mi Jin Kim, Jung Bin Lee

Department of Forensic Medicine, Seoul National University College of Medicine, Korea.

Abstract

A duplex PCR reactions were designed for two pentameric (Penta D, Penta E) short tandem repeats (STR) loci to study the allele frequencies and the applicability of genetic variation in these loci to forensic case works. For 323 unrelated Koreans DNA was extracted using Qiagen DNA blood kit. The fluorescently labeled amplified products were analyzed by 8M urea, 5% long range gel electrophoresis in ABI-377 sequencer and followed data analysis by Genescan Analysis and Genotyper DNA fragment analysis software. The PD, PIC and PE were above 0.92, 0.76 and 0.66, respectively. No deviation from Hardy-Weinberg equilibrium was observed of Penta D locus (p=0.35, observed number>1).

Key Words: Pentameric, STR, Penta D, Penta E, Korean