Quadruplex PCR for 8 Autosomal STR Loci in Korean.

Zhang, Yong Ji; Kim, Ki Beom; Shin, Chang Ho; Lee, Soong Deok; Lee, Yoon Seong; Lee, Jung Bin

Original article

Korean Journal of Legal Medicine 2001;25(1):17-28.
Published online May 31, 2001.

Quadruplex PCR for 8 Autosomal STR Loci in Korean.

Yong Ji Zhang, Ki Beom Kim, Chang Ho Shin, Soong Deok Lee, Yoon Seong Lee, Jung Bin Lee

Department of Forensic Medicine, Seoul National University College of Medicine.

Abstract

Two quadruplex PCR reactions were designed for 7 tetrameric (D3S2406, D4S2368, D5S818, D7S821, D9S925, D13S317, D19S253) and one trimeric (D6S1043) short tandem repeats loci to study the allele frequency and the applicability of genetic variation in these loci in forensic case works. For 310 unrelated Koreans DNA was isolated from peripheral blood using phenol/chloroform method. Quadruplex I was consisted of D4S2368, D6S1043, D7S821, D9S925 and quadruplex II D3S2406, D5S818, D13S317, D19S253. The amplified products were analyzed by 5%polyacrylamide gel electrophoresis followed by silver staining. The heterozygosity in each loci ranged 92.91-66.13%, and PD(Power of Discrimination) was above 0.85 in each loci. Every loci except D6S1043 followed hardy-Weinberg expectation. The cumulative PI was low as 1.65x10-10. Two mutations were noted, one in D19S253 and the other in D9S925 among 234 gametes. With these results above eight STR loci studied here proved to be highly polymorphic enough to be used in forensic field. This study provides valuable population data in these loci for Korean.

Key Words: STR, D4S2368, D6S1043, 7S821, D9S925, D3S2406, D5S818