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Korean Journal of Legal Medicine 2007;31(1):30-35.
Published online May 31, 2007.
Application of Differex(TM) system and FTA(R) technology to separation of sperm DNA from mixed stains.
Jin Hee Kim, Dong Sub Lee, Eung Soo Kim, Nam Ye Kim, Jong Min Jung, Young Ae Lee, Myun Soo Han
1DNA Analysis Division, National Institute of Scientific Investigation, Seoul, Korea. jinee@nisi.go.kr
2Forensic Medicine Division, National Institute of Scientific Investigation, Eastern District, Korea.
Abstract
Specimens from sexual crimes are generally mixed stains consisted of sperm cells(from suspect) and virginal cells(from victim). We have combined two new methods - Differex(TM) system and FTA(R) technology- to overcome shortcomings of method that has been used before to separate sperm DNA from mixed stains. This methods have shown additional benefits and similar quality than using the only Differex(TM) system to the experiment. The result of our experiment represents the possibility that Differex(TM) system and FTA(R) technology would be useful methods for DNA analysis related to sexual crimes because this system can save time, labor and contamination for experiments.
Key Words: Sexual crimes, Mixed stain, Differex(TM) system, FTA(R) technology
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