Three Allele Pattern Detected by STR Typing.

Yi, Jina; Zhang, Ai Hua; Seo, Seung Bum; Kim, Hye Yeon; Lee, Soong Deok

Case Report

Korean Journal of Legal Medicine 2008;32(2):118-123.
Published online November 30, 2008.

Three Allele Pattern Detected by STR Typing.

Jina Yi, Ai Hua Zhang, Seung Bum Seo, Hye Yeon Kim, Soong Deok Lee

Department of Forensic Medicine and Institute of Forensic Medicine, Seoul National University College of Medicine, Seoul, Korea. sdlee@snu.ac.kr

Abstract

STR typing for several loci has been regarded as a standard method for individual identification. The result is rather simple and easy to be interpreted. For each loci one allele for homozygote and two different alleles for heterozygote are expressed as simple peaks. Many peaks more than two suggest unusual situations such as contamination. We experienced two cases of three alleles in two separate samples. Among 15 STR loci typed using AmpFlSTR.. Identifiler.. Kit(Applied Biosystems, Foster City, CA) and Powerplex..16 system (Promega, Madison, WI), three alleles were noted in D8S1179 locus in one sample, in D21S11 locus in another sample. We sequenced all the three amplified alleles through cloning to reveal the structure, and amplified nearby regions to check the extent of three alleles. We present the results here together with review on many alleles in one sample.

Key Words: STR, Individual identification, Three allele, D8S1179, D21S11