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Korean J Leg Med > Volume 42(4); 2018 > Article
Korean Journal of Legal Medicine 2018;42(4):126-140.
DOI: https://doi.org/10.7580/kjlm.2018.42.4.126    Published online November 30, 2018.
Analysis of the Absorbance Pattern of Postmortem Blood Sample Using Spectrometer.
Joo Young Na, Jong Tae Park
1Biomedical Research Institute, Chonnam National University Hospital, Gwangju, Korea.
2Emergency Medical Center, Chonnam National University Hospital, Gwangju, Korea.
3Department of Forensic Medicine, Chonnam National University Medical School, Gwangju, Korea. jtpark@jnu.ac.kr
Abstract
The diagnosis of cause of death (COD) or estimation of postmortem interval (PMI) is hard to perform using postmortem blood or other bodily fluids due to various biochemical changes that occur during the agonal phase or after death. To solve these problems, new paradigms and new analytical methods are needed. In this study, postmortem blood was fractionated with specific gravity 1.021, 1.029, 1.038, and 1.045, and the absorbance patterns of each sample of the 131 total cases (12 kinds of COD) were analyzed using a spectrometer. The absorbance was grouped into 9 patterns (ABS pattern 1 to 9) according to the wave length and the signal intensity. These patterns of postmortem blood were found to be distinctly different from the absorbance pattern of fresh blood. The analysis of ABS patterns is useful for the diagnosis of deaths due to acute or rapid bleeding, fire death, drowning and, in some cases, poisoning, but is not useful for the estimation of PMI.
Key Words: Spectrometry, Absorbance pattern, Cause of death, Postmortem interval
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