| DNA Typing Analysis for the Identification of Bone Fragments from Sampoong Disaster |
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Yong Suk Nam , Hee Suk Lee , Hye Rin Lee , Hee Sun Kim , Kyung Hoon Kim , Juck Joon Hwang |
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The Institute of Legal Medicine, Korea University Department of Legal Medicine, Korea University, College of Medicine |
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| Abstract |
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A DNA typing was performed to identify decomposed body remains from Sampoong Department mass disaster in June 1995. These body parts include bone fragment, skin tissue, hairs, from which the extracted DNAs were highly degraded. Two VNTR loci, 4 STR loci, and amelogenin gene were chosen for AMP -FLP, and mtDNA sequence analysis for the confirmation of maternal relationship. The results of AMP-FLP of the selected polymorphic loci showed different sucess rate for PCR. D1S80 and D17S5 loci were amplified successfully from 64.5%, and 67% of the samples, respectively. HUMTHO1, HUMCSF1PO, and HUMPTPOX loci were ampified successfully from 90.3% of the samples each. HUM A CTBP2 and amelogenin was amplified in 87% of the cases submitted. The DNA types of 33 remains were compared with those of 81 bereaved families consisting of 173 member. Thirty three samples were reduced to 28 in numbers according to results of the same DNA types. Among them, the DNA types of 15 remains matched with those of bereaved families and the identified remains were reconfirmed by amelogenin sex typing and mitochondrial DNA sequence analysis. The others were not identified a family by failures of PCR amplification or non-matching of DNA types. Also it is confirmed that one hair sample should be artifitia1 by non-digestion of protease and another be animal bone by result of dot blotting with human Alu probe. Our results indicate that multiplex PCR systern consisting of several STR loci like HUlMCSF1PO, HUMTPOX, and HUMITHOl is more effective for the identification of highly decomposed human remains from mass disaster |
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