| Individual Identification form Mixed Blood by Polymerase Chain Reaction Amplification for Variable Number of Tandem Repeats Loci |
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Jong Tae Park , Chan Choi , Sang Woo Juhng |
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Department of Pathology and Department of Forensic Medicine, Chonnam University Medical School Laboratory of Molecular Cytogenetics, National Institute of Environmental Health Science, North Carolina, U.S.A. |
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| Abstract |
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Genetic loci that contain a variable number of tandem repeats (VNTR) could be suc- cessfully amplified from a ver y small amount of genomic DNA by the polymerase chain reaction(PCR) and have been applied to individual identification in forensic field. Forensic samples are sometimes not a sample of one individual, but mixed sample, such as blood, hairs, and vaginal fluid with sperm. Even though very small amount of sample is mixed to the large one, the small fraction of the mixture is amplified by PCR. But sometimes it could not be detected in electrophoresed agarose gel with ethidium bromides Iaining, because the PCR products of small fraction are less amount rather than that of large fraction of mixed sample and because of the limitation of the sensitivity of ethidium bromide staining in agarose gel. We investigated to search the detectable limitation of the PCR products of mixed sample with various ratio, experimentally. The sample were mixed with various ratio, amplified by polymerase chain reaction, electroplioresed, and stained with ethidium bromide. We can detect the small fraction of mixed sample from the 50% to the 5.9% or 3.0% of blood mixture and to the 0.8% of DNA mixture. In Southern blot, we can detect the small fraction in all ratio of blood mixture, to the 0.26% which is the smallest ratio of mixture in this experiment. From the results, in samples which have a possibilit y of mixed sample, the southern blot analysis is necessary to increase the sensitivity of DNA fingerprint. |
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