Typing of DNA Extracted from Cigarette Butts for Individual Identification

, Kyong kyue Yoon; ,  Kyung Jin Shin; ,  Chong Youl Kim; ,  Juck Jun Hwang

Original article

Korean Journal of Legal Medicine 1995;19(1):5-16.

Kyong kyue Yoon , Kyung Jin Shin , Chong Youl Kim , Juck Jun Hwang

Department of Oral Diagnosis, Dental College, Yonsei University Department of Legal Medicine, Korea University, College of Medicine

Abstract

Cigarette butts from 5 smokers were gathered and then, placed in room temperature for 1, 3, 5, 7, 15 days. The possible use of the cigarette butts for individual identification was evaluated in sex determination, amplification of D1S80 locus, polymorphisms of HLA -DQA1 gene from the extracted DNA. 1. DNA extraction was possible in cigarette butts left in room temperature for 15 days, so it can be applicable to individual identification by polymerase chain reaction (PCR). 2. Amplification of X-Y homologous amelogenin gene by PCR made it possible to identify the sex in saliva stains (cigarette butts). 3. Amplification of D1S80 locus can be acquired from adding the bovine serum albimine and hot start PCR procedures from forensic samples such as saliva stains (cigarette butts), so the AMP-FLPs examining is possible. 4. Genotype could be determined simply and rapidly using Amplitype TMHLA-DQα. forensic kit in examining the HLA -DQA1 gene. From the investigation, DNA extraction, sex determination, amplification of D1S80 locus, polymorphisms of HLA-DQA1 gene were successfully done even though the cigarette butts were left for 15 days at room temperature. Therefore cigarette butts are highly reliable and applicable as molecular biological samples for individual identification.

Key Words: Cigarette butt, DNA, PCR, X -Y homologous amelogenin gene, D1S80, HLA-DQA1