Effect of Adenine on Discocyte-echinocytic and Discocyte-stomatocytic Transformation of Human Red Cell |
Jin Choe , Jung Young Lee , Jin Sung Bang |
Department of Pathology, Catholic Medical College, Seoul, Korea |
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Abstract |
Since it has been postulated that the maintenance of a biconcave shape of red blood cell is a property of the membrane itself, it has been studied the mechanism for maintenance the shape of the mammalian red blood cells. Recently, we demonstrated that adenine is effective in the prolongation of the maintenance of the normal red cell shape during cell preservation, and that some of crenator and cupformer transformed the red cell shape.
In this paper the effect of adenine on discocyte-echinocytic and discocyte-stomatocytic transformation of human red cell was studied. The experimental groups were divided into adenine-pretreated and non-pretreated groups. In non-pretreated group, red cells and their ghosts were treated with crenators such as furosemide (10 mM), dipyridamol (0.05 mM), dinitrophenol (1 mM) and saponin (10 mg/ 100 ml), and cupformers such as chlorpromazine((). 15 mM), phenothiazine(0. 15 mM), Triton-X 100 (51.11/100 ml) and dl-propranolol (5 mM).
In adenine-pretreated group, red cells and ghosts were previously treated with adenine, and incubated with above drugs. All the specimens were fixed, dehydrated, dried, coated and then examined under scanning electron microscope.
The results were as follows:
1. The crenators transformed the red cells and ghosts into echinocytes. The ghosts
were transformed more prominently than the red cells.
2. The cupformers transformed the red cells into stomatocytes. The ghosts were transformed more prominently. Chlorpromazine transformed the ghosts somewhat differently and converted crenated surface to smooth surface.
3. The effect of adenine on echinocytic and stomatocytic transformation of the human red cells and ghosts was indistinct.
These results reveal that the transformations of human red cells induced by crenators and cupformers are closely related to the structure of cell membrane based on the fluid mosaic theory. |
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