Allele Frequency Distributions with an Analysis of Allelic Sequence Variations for HumFIBRA/FGA and D21S11 Loci in Korean.

Kim, Gil Ro; Kim, Yoon Sin; Lee, Hae Lin; Koo, Tai Wan; Lee, Hye Seung; Han, Gil Ro; Hwang, Juck Joon

Original article

Korean Journal of Legal Medicine 1998;22(1):1-12.
Published online May 31, 1998.

Allele Frequency Distributions with an Analysis of Allelic Sequence Variations for HumFIBRA/FGA and D21S11 Loci in Korean.

Gil Ro Kim, Yoon Sin Kim, Hae Lin Lee, Tai Wan Koo, Hye Seung Lee, Gil Ro Han, Juck Joon Hwang

Department of Legal Medicine, Korea University College of Medicine, Korea.

Abstract

Allele-and genotype frequencies of the two short tandem repeat (STR) loci, HumFGA and D21S11, were determined in Korean population(n=196). DNA typing was accomplished by applying fluorescence-labeled PCR products and a differently labeled sequenced allelic ladders, followed by automated analysis using ABI 377 automatic sequencer and GeneScan 2.02 software. Prior to typing, allelic ladder of each locus was constructed with a combination of all alleles occuring from the population sample. A total of 15 alleles and 48 genotypes with the heterozygosity of 0.854 for HumFGA, and 12 alleles and 33 genotypes with the heterozygosity of 0.787 for D21S11 are observed in a population of 196 genetically unrelated individuals. No deviations from Hardy-Weinberg equilibrium were observed(p=0.753 for HumFGA, p=0.262 for D21S11). The data presented here (power of discrimination and average power of exclusion) show that both STR Loci, HumFGA and D21S11, are very informative for individualization from criminal evidences, and are also useful for parentage testing.