| Mixture Patterned Short Tandem Repeat Profile in a Perimortem Transfused Patient. |
|
Hee Jin Seo, Sohee Cho, Ji Hyun Lee, Sung Hee Lyoo, Sohyung Park, Soong Deok Lee |
1Department of Forensic Medicine, Seoul National University College of Medicine, Seoul, Korea. sdlee@snu.ac.kr
2Institute of Forensic Science, Seoul National University College of Medicine, Seoul, Korea.
3Medical Examiner's Office, National Forensic Service, Wonju, Korea. |
|
|
| Abstract |
|
Recently, it has been reported that transfused patients can generate admixture-like genetic profiles. As genetic material of the donor can survive for a reasonable time after transfusion, the recipient's genomic DNA is likely to have a mixture pattern. An autopsy case of a man transfused perimortem generated a mixture patterned short tandem repeat profile. Notably, the patient was transfused mostly with nuclear-deficient cells, limiting the donor genetic material available for the recipient. As a result, mixture-like patterns were observed consistently, regardless of change in input DNA content; the sample DNA content, which was serially diluted, ranged from 1 ng to 0.0625 ng. The distributions of foreign peaks appeared to be irreproducible, showing stochastic behaviors throughout the genotyped results. This study suggests that a cautious approach is required when genotyping of a patient who has undergone recent transfusion. One must consider the possibility of obtaining a mixture patterned profile in such patients, and therefore, choose parenchymal organs or tissues for reliable results. |
| Key Words:
Perimortem, Blood transfusion, DNA profiling, Microsatellite repeats |
|
PDF Links
Full text via DOI
Download Citation
