| Significance of Shared DNA Fingerprints between a Putative Father and Child Detected by Multi-locus pV47-2 Probe in Paternity Test |
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Yong Suk Nam , Hye Rin Lee , Hee Sun Kim , Hee Suk Lee , Juck Joon Hwang |
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Korea University Institute of Legal Medicine Department of Legal medicine, Korea University, College of Medicine |
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| Abstract |
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Conventional paternity index and probability of paternity can not be calculated when probe that hybridize to multiple VNTR loci because alleles are not ascertained to specific loci, Therefore, an alternate method based upon the chance of band-sharing is used to get paternity index and probability of paternity in this study using multi-locus pV47-2 probe. From 57 parentage cases of Korean pedigree we found 12.08, 11.86, and 12.31 of average DNA fingerprints present in mother, father and child., respectively. Among 614 DNA bands present in 57 children, 7 bands arc not assignable to neither of the parents and overall mutation rate is 0.0138 per band in an individual. Average number of bands shared between mother and father is 1.71, with mean band-sharing coefficiency of 0.1798. All bands present in the child's pattern but absent in the mother's are 5.14 on average as obligatory paternal band, but a child are transmitted 5, 47 from mother. In 10 non-parentage cases, however, a child shared bands of 1.20 on average with a putative father, whereas shared bands of 5.60 with a biological mother. In the paternity test of a child having 3 bands of obligator paternal origin, paternity index(PI) and probability of paternity(PP) are calculated with the band-sharing coefficiency of 0.18 as 21.4, and 95.4%, respectively. These results suggest that RFLP analysis using multi-locus pV47-2 probe could identify with virtual certainty one particular man as the biological father of a child. |
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