An Experimental Study on the Effect of Several Conditions to the Preliminary Examinations of Blood Stain

, Shin Mong Kang

Original Article

Korean Journal of Legal Medicine 1984;8(Suppl DB Error: syntax error):13-21.

Department of Legal Medicine, Korea University, College of Medicine, Institute of Legal Medicine, Korea University, Seoul, Korea

Abstract

Of all analytic tests for various substances, that for blood stain is the most important from the viewpoint of legal medicine. Among blood stain preliminary examinations, which are basic for the blood stain determination, the benzidine, leukomalachite green, and phenolphthalein tests have been commonly used. Especially, the benzidine test was most frequently had recourse for its high sensitivity and ease to be carried out. Since the ban of this method for carcinogenic substance, however, there have been no comparable tests and the leukomalachite green and phenolphthalein tests seem to be the conventional methods at present. So, the author studied on the sensitivities of these blood stain preliminary examinations by lapsed time in shady and sunny place. Also, the sensitivities of preliminary examinations in other several conditions and the materials which react as positive to preliminary examinations were examed. The results could be summarized as follows. I. The sensitivity to blood dilution of benzidine test was 120,000~160,000 times, and that of leukomalachite green and. phenolphthalein tests was 8,000 times. But after blood stain formation, the sensitivities dropped to about half in all preliminary tests. 2. By lapse of time, the sensitivities of all preliminary examinations dropped simultaneousely 3. The sunlight on the blood stain does not affect on the sensitivities of preliminary examinations which performed this experiment until 4 months. 4. Ultraviolet ray or infrared ray on the b100d stain and culture or decomposition of blood did not affect on the sensitivities of preliminary examinations which performed this experiment. 5. The juice of gimchi, made of garlic, onions and other raw materials, reacted as positive to benzidine test.